| 1 | James Carter & The Prisoners - | Po Lazarus | 4:30 | |
| 2 | Harry McClintock - | Big Rock Candy Mountain | 2:17 | |
| 3 | Norman Blake (2) - | You Are My Sunshine | 4:26 | |
| 4 | Alison Krauss - | Down To The River To Pray | 2:53 | |
| 5 | Soggy Bottom Boys - | I Am A Man Of Constant Sorrow (Radio Station Version) | 3:13 | |
| Lead Vocals, Guitar - Dan Tyminski | ||||
| 6 | Chris Thomas King - | Hard Time Killing Floor Blues | 2:01 | |
| 7 | Norman Blake (2) - | I Am A Man Of Constant Sorrow (Instrumental) | 4:30 | |
| 8 | Whites, The - | Keep On The Sunny Side | 3:35 | |
| 9 | Gillian Welch & Alison Krauss - | I'll Fly Away | 3:57 | |
| 10 | Gillian Welch & Alison Krauss & Emmylou Harris - | Leave Nobody But The Baby | 1:58 | |
| 11 | Sarah Peasall & Hannah Peasall & Leah Peasall - | In The Highways | 1:36 | |
| 12 | Cox Family, The - | I Am Weary (Let Me Rest) | 3:16 | |
| 13 | John Hartford - | I Am A Man Of Constant Sorrow (Instrumental) | 2:34 | |
| 14 | Ralph Stanley - | O Death | 3:21 | |
| 15 | Soggy Bottom Boys - | In The Jailhouse Now | 3:36 | |
| Vocals - Tim Blake Nelson | ||||
| 16 | Soggy Bottom Boys - | I Am A Man Of Constant Sorrow (With Band) | 4:18 | |
| Lead Vocals, Guitar - Dan Tyminski | ||||
| 17 | John Hartford - | Indian War Whoop | 1:30 | |
| 18 | Fairfield Four - | Lonesome Valley | 4:08 | |
| 19 | Stanley Brothers, The - | Angel Band | 2:18 | |