| 1 | Amos Milburn - | Down The Road Apiece | 2:56 | |
| Producer - Eddie Mesner | ||||
| 2 | Jackie Brenston - | Rocket 88 | 2:47 | |
| Producer - Sam Phillips (2) | ||||
| 3 | Robert Johnson - | Preachin' Blues (Up Jumped The Devil) | 2:49 | |
| Producer - Don Law | ||||
| 4 | Muddy Waters - | Rollin' Stone | 3:04 | |
| Producer - Leonard Chess | ||||
| 5 | Jimmy Rogers - | Goin' Away Baby | 2:40 | |
| Producer - Leonard Chess | ||||
| 6 | Leadbelly - | The Midnight Special | 3:02 | |
| Featuring - Golden Gate Quartet, The | ||||
| 7 | Clarence 'Gatemouth' Brown* - | Okie Dokie Stomp | 4:05 | |
| Producer - Clarence Brown , Jim Bateman | ||||
| 8 | Clifton Chenier - | Ay-Te Te Fee | 2:39 | |
| Producer - Bumps Blackwell* | ||||
| 9 | Professor Longhair & His Shuffling Hungarians - | Mardi Gras In New Orleans | 2:50 | |
| Producer - Ahmet Ertegun , Herb Abramson | ||||
| 10 | Little Richard - | Good Golly Miss Molly | 2:08 | |
| Producer - Bumps Blackwell* | ||||
| 11 | Billie Holiday - | He's Funny That Way | 2:37 | |
|
Producer -
John Hammond
Saxophone [Tenor] - Lester Young | ||||
| 12 | John Lee Hooker - | I'm In The Mood | 3:04 | |
| Producer - Bernie Besman* | ||||
| 13 | Bob Marley & The Wailers - | Jah Is Mighty | 2:24 | |
| Producer - Lee Perry | ||||
| 14 | Hank Williams - | You Win Again | 2:34 | |
| Producer - Fred Rose | ||||
| 15 | Ike & Tina Turner - | I Can't Believe What You Say | 1:59 | |
| Producer - Ike Turner | ||||
| 16 | BB King* - | Everyday I Have The Blues | 2:46 | |
| 17 | T-Bone Walker - | (They Call It) Stormy Monday | 2:59 | |
| 18 | Howlin' Wolf - | Moanin' At Midnight | 2:39 | |
| Producer - Joe Bihari | ||||
| 19 | Blind Willie McTell - | Talkin' To Your Mama | 3:05 | |
| Producer - Fred Mendelson* | ||||
| 20 | Clarence 'Bon Ton' Garlow* - | Bon Ton Roulet | 2:49 | |
| Producer - Eddie Mesner | ||||
| 21 | Aaron Neville - | Tell It Like It Is | 2:39 | |
| Producer - George Davis (2) | ||||
| 22 | Albert King - | That's What The Blues Is All About | 3:49 | |
| Producer - Allen Jones* , Henry Bush | ||||
| 23 | Irma Thomas - | Ruler Of My Heart | 2:32 | |
| Producer - Allen Toussaint | ||||
| 24 | Otis Redding - | Pain In My Heart (Alt. Take) | 2:20 | |
| 25 | Booker T & The MGs* - | Baby, Scratch My Back | 3:01 | |
| Producer - Booker T Jones* , Steve Cropper | ||||
| 26 | Al Green - | Take Me To The River | 4:23 | |
| Producer - Willie Mitchell | ||||