Jackie-O Motherfucker began as a duo consisting of multi-instrumentalist Tom Greenwood and saxophonist Nester Bucket. The group has had more than forty rotating members drawn from the U.S. experimental scene. As of 2006, the core of the group is founding member Tom Greenwood.

current line-up (2008) :
Tom Greenwood (voice/guitar)
Honey Owens (bass)
Danny Sasaki (drums)
Nick Bindeman (guitar)
Dana Valatka (electronic percussion, voice)
Brooke Crouser

Former members (amongst others) :
Natalie Mering (voice, guitar)
Nester Bucket
Joshua Stevenson
Adam Forkner
Fluffy Erskine
Jessie Carrot
Theo Angell
Jef Brown
John Flaming
Michael Henerikson